首页> 外文OA文献 >Two beta-glycanase genes are clustered in Bacillus polymyxa: molecular cloning, expression, and sequence analysis of genes encoding a xylanase and an endo-beta-(1,3)-(1,4)-glucanase.

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Two beta-glycanase genes are clustered in Bacillus polymyxa: molecular cloning, expression, and sequence analysis of genes encoding a xylanase and an endo-beta-(1,3)-(1,4)-glucanase.

机译：在聚粘芽孢杆菌中有两个β-聚糖酶基因：分子克隆，表达和编码木聚糖酶和内切β-（1,3）-（1,4）-葡聚糖酶的基因的序列分析。

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Two genes, xynD and gluB, encoding a xylanase and an endo-beta-(1,3)-(1,4)-glucanase (lichenase) from Bacillus polymyxa have been cloned and expressed in Escherichia coli and Bacillus subtilis. A sequenced DNA fragment of 4,466 bp contains both genes, which are separated by 155 bp. The xynD and gluB genes encode proteins of 67.8 kDa (XYND) and 27 kDa (GLUB). Two peptides with molecular masses of 62 and 53 kDa appear in cell extracts of E. coli and culture supernatants of B. subtilis clones containing the xynD gene. Both peptides show xylanase activity in zymogram analysis. The XYND enzyme also shows alpha-L-arabinofuranosidase activity. The XYND peptide and the xylanase XYNZ from Clostridium thermocellum (O. Grépinet, M. C. Chebrou, and P. Béguin, J. Bacteriol. 170:4582-4588, 1988) show 64% homology in a stretch of about 280 amino acids.

机译：已经克隆了来自多粘芽孢杆菌的编码木聚糖酶和内切β-（1,3）-（1,4）-葡聚糖酶（地衣酶）的两个基因xynD和gluB，并在大肠杆菌和枯草芽孢杆菌中表达。 4466 bp的测序DNA片段包含两个基因，它们之间相距155 bp。 xynD和gluB基因编码67.8 kDa（XYND）和27 kDa（GLUB）的蛋白质。大肠杆菌的细胞提取物和含有xynD基因的枯草芽孢杆菌克隆的培养上清液中出现了两种分子量分别为62和53 kDa的肽。两种肽在酶谱分析中均显示木聚糖酶活性。 XYND酶也显示出α-L-阿拉伯呋喃糖苷酶活性。来自热纤梭菌的XYND肽和木聚糖酶XYNZ（O.Grépinet，M.C.Chebrou和P.Béguin，J.Bacteriol.170：4582-4588，1988）在约280个氨基酸的片段中显示64％的同源性。

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Gosalbes, M J; Pérez-González, J A; González, R; Navarro, A;
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年度 1991
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1. Two beta-glycanase genes are clustered in Bacillus polymyxa: molecular cloning, expression, and sequence analysis of genes encoding a xylanase and an endo-beta-(1,3)-(1,4)-glucanase. [J] . M J Gosalbes, J A Pérez-González, R González, Journal of bacteriology . 1991,第23期

机译：两个β-聚糖酶基因聚集在芽孢杆菌中：分子编码，木聚糖酶和内切β-（1,3）-（1,4）-葡聚糖酶的基因的克隆，表达和序列分析。
2. Molecular cloning, expression, and characterization of endo-beta-1,4-glucanase genes from Bacillus polymyxa and Bacillus circulans. [J] . S D Baird, D A Johnson, V L Seligy Journal of bacteriology . 1990,第3期

机译：来自多粘芽孢杆菌和圆形芽孢杆菌的内切β-1,4-葡聚糖酶基因的分子克隆，表达和表征。
3. Molecular Cloning and Expression of a Xylanase Gene from Bacillus polymyxa in Escherichia coli [J] . Robert C. A. Yang, C. Roger MacKenzie, Doris Bilous, Applied and Environmental Microbiology . 1988,第4期

机译：多粘芽孢杆菌木聚糖酶基因的分子克隆与表达
4. MOLECULAR CLONING, EXPRESSION AND DNA SEQUENCE ANALYSIS OF A GENE ENCODING AN OUTER MEMBRANE PROTEIN OF BRACHYSPIRA (SERPULINA) PILOSICOLI [C] . D.P. Alt, D.J. Trott, T.B. Stanton Congress of the International Pig Veterinary Society . 2000

机译：一种编码Brachyspira（Serpulina）pilosicoli的外膜蛋白的基因的分子克隆，表达和DNA序列分析
5. The cloning, comparison, and expression of three family G endo beta-1,4-xylanase genes of the maize fungal pathogen Cochliobolus carbonum and analysis of their importance for pathogenicity on maize [D] . Apel, Patricia Carlene. 1996

机译：玉米真菌病原菌Cochliobolus carbonum的三个G族内切β-1,4-木聚糖酶基因的克隆，比较和表达及其对玉米致病性的重要性分析
6. Two beta-glycanase genes are clustered in Bacillus polymyxa: molecular cloning expression and sequence analysis of genes encoding a xylanase and an endo-beta-(13)-(14)-glucanase. [O] . M J Gosalbes, J A Pérez-González, R González, 1991

机译：在聚粘芽孢杆菌中有两个β-聚糖酶基因：分子克隆表达和编码木聚糖酶和内切β-（13）-（14）-葡聚糖酶的基因的序列分析。
7. Molecular cloning, expression and nucleotide sequence of the endo-beta-1,3-1,4-d-glucanase gene from Bacillus licheniformis Predictive structural analyses of the encoded polypeptide [O] . Jorge LLOBERAS, Josep A. PEREZ-PONS, Enrique QUEROL 1991

机译：Endo-Beta-1,3-1,4-D-葡聚糖酶基因的分子克隆，表达和核苷酸序列来自编码多肽的芽孢杆菌预测结构分析
8. Molecular Cloning, Characterization, and Regulation of a 'Pseudomanas pickettii'PKO1 Gene Encoding Phenol Hydroxylase and Expression of the Gene in 'Pseudomonas aeruginosa' PAO1c [R] . Kukor, J. J., Olsen, R. H. 1990

机译：“铜绿假单胞菌”paO1c中苯酚羟化酶'pseudomanas pickettii'pKO1基因的克隆，表征和调控及其基因的表达

Two beta-glycanase genes are clustered in Bacillus polymyxa: molecular cloning, expression, and sequence analysis of genes encoding a xylanase and an endo-beta-(1,3)-(1,4)-glucanase.

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